Device

Part:BBa_K4611008:Design

Designed by: Tuo Wang   Group: iGEM23_CJUH-JLU-China   (2023-10-03)


His-SUMO-LwaCas13a under T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3367
    Illegal XbaI site found at 47
    Illegal PstI site found at 3388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3367
    Illegal NheI site found at 151
    Illegal PstI site found at 3388
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3367
    Illegal BglII site found at 341
    Illegal BglII site found at 1823
    Illegal BglII site found at 2033
    Illegal BglII site found at 2300
    Illegal BglII site found at 3065
    Illegal BamHI site found at 532
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3367
    Illegal XbaI site found at 47
    Illegal PstI site found at 3388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3367
    Illegal XbaI site found at 47
    Illegal PstI site found at 3388
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Synthetic; the sequence of LwaCas13a is from Leptotrichia wadeii genome, but is human codon optimized. Original gene is from Genbank:Leptotrichia wadei F0279.

References